The importance of disposables for a (q)PCR reaction is often underestimated. Money spent on kits, polymerases, primers, dyes, the optimization of reaction components and (q)PCR cycler calibration should be supported by superior BIOplastics (q)PCR tubes, strips and plates instead of the often used less qualified or even inferior products. Keywords here are: Reproducible and trustworthy results. Enjoy the benefits and ease of use of superior quality (q)PCR vessels which all have the same raw material product compositions. Combine this with the extreme uniform wall thickness of the products and you actually have standardized and automatically improved the reproducibility of your results by excluding variables in uniformity and material composition.
BIOplastics has assembled a range of products which are fully interchangeable with each other.A (q)PCR process optimized in one of the BIOplastics tubes, strips or plates can be transferred to any other BIOplastics tube, strip or plate. The fact that BIOplastics offers products for ALL BRANDS and models of PCR and qPCR cyclers enables the end-user to optimize the (q)PCR process using interchangeable plastics with the same properties. Using the same protocol on a different cycler is easily done by selecting the designated BIOplastics product for that model of cycler. No need for re-optimizing the whole process since BIOplastics’ plastics characteristics are exactly the same for the whole range of the (q)PCR product line. Thermal performance difference between cyclers, cycler models, and brands can be exactly determined using CYCLERtest® calibration service or CYCLERtest® DRIFTCON® temperature calibration tools. See also “Normalizing Standard Operation Procedures (SOP) for (q)PCR applications. Did you optimize your (q) PCR process in tube strips and need more or less capacity? Just start using BIOplastics plates, single tubes or even 384 well plates without the requirement of adjusting your protocol.
BIOplastics focus provides maximum mould and manufacturing accuracy, while precise product specifications result in superior and reproducible products. BIOplastics’ (q)PCR vessels have an average wall thickness of 0.30 mm and a maximum tolerance of 0.05 mm. These extremely tight tolerances lead to extraordinary uniform and reproducible products. Consequently, reproducible melting and high resolution melting curves, typically used in the post qPCR process, are generated using BIOplastics extremely uniform products. HRM curves can be normalized, i.e. “cycler fingerprint” adjusted, by using CYCLERtest®‘cycler calibration services and calibration tools. (www.cyclertest.com)
Shift of melting curves of one amplified fragment in qPCR. Shift caused by difference in qPCR plates as well as un-uniformity of the qPCR cycler.
qPCR signal/noise ratios can be significantly increased by using frosted qPCR tubes, strips
and plates. Frosted products will generate higher signal/noise ratios (up to 40%) compared
to transparent ones. By using frosted products both content viewability and high signal to noise ratio are achieved. White products are superior for enhancing signal/noise ratios. White products greatly enhance signal/noise ratios (up to 90%) compared to transparent ones and up to 50% compared to frosted products. Possible block pollution of dyes, differences in coatings, and inconsistent coatings can influence qPCR signals. White products will eliminate these influences while frosted products will reduce the inconsistencies when compared to natural transparent products. Micro-particles, by nature, increase signal to noise ratios in Real-Time PCR applications. BIOplastics BPLPM technology incorporates micro-particles that increase signal to noise ratios in Real-Time PCR applications.
| Natural Transparent | 100 | 100 |
| Light Frosted | 125 | 70 |
| Light Frosted + BPLPM | 140 | 50 |
| Frosted | 140 | 50 |
| Frosted + BPLPM | 150 | 30 |
| White | 180 | < 2 |
| white + BPLPM | 185 | < 1 |