Europe based BIOplastics BV is the innovative high quality injection moulding manufacturer of (q)PCR
consumables supplying the molecular (diagnostic) (q)PCR marketplace (MDX). BIOplastics with its sole
headquarter and manufacturing facility in Landgraaf, The Netherlands is a member of the privately owned
BIOzym Holding group, which is comprised of CYCLERtest BV, GENO-tronics BV, BIOzymTC BV, BPCTi Inc.
(USA), Hendrikx Ltd. (China) and CelsiusLabs. BIOplastics offers the most superior range of (q)PCR plastics
globally. BIOplastics pursues the vision to design and manufacture plastic consumables in particular for use in
MDX and related application fields as well as to supply MDX kit manufacturers. BIOplastics superior quality
and highly reproducible consumables allows ease of use with maximum reproducibility of results.
BIOplastics products are, in addition to being integrated into MDX kits, offered through a worldwide network
of selected distributors and resellers.
Michael T. Hendrikx. CEO BIOplastics BV
BIOplastics BV Strategic Views
The molecular (diagnostics) marketplace is rapidly growing and requires superior, independent and complete
supply of (q) PCR disposables. These demands require generation of accurate, consistent, and reproducible
results to manage and reduce variations in the (q)PCR process.
BIOzym Holding, the Netherlands, the parent company of BIOplastics and its sister company CYCLERtest,
support and commit to these demands and strategically organize all members to develop new products whose
end result is to comply with these "manage and reduce variations in the (q)PCR process” demands.
While CYCLERtest supplies independent ISO17025 accredited (q)PCR cycler tools and calibrations for all
models and brands of (q)PCR cyclers, BIOplastics manufactures Extreme Uniform q(PCR) disposables, and has
been able to reduce to the use of just two products for those who require the lowest variability, the number of
to be used products to a minimum of two.
BIOplastics is proud to be categorized as global innovative leader and we are obligated and eager to
maintain this position.
|(q)PCR tubes/strips & plates||Special blend of polypropylene||Anti-static tubes||Maximum sample recovery|
|Tubes non sticking for biomolecules||Low copy detection|
|Flexible non cracking tubes||No loss of sample|
|Uniform wall thickness||Uniform heating||Consistent reaction conditions|
|No risk of cracking||No loss of sample|
|Minimize evaporation||Consistent reaction conditions|
|Uniform closure part tube/strip/plate||Uniform closure & interchangeability||Consistent reaction conditions|
|Thin wall lower part tube||Maximal heat transfer||Optimal reaction conditions|
|Thick wall upper part tube||Minimal evaporation||Consistent reaction conditions|
|Sealing band in cap||Minimal evaporation||Consistent reaction conditions|
|Clever design cap||Easy opening and closing||“Finger” friendly and reduce RSI|
|Minimal evaporation||Consistent reaction conditions|
|Wide area indented caps||No “finger touch” while closing||Highly reproducible results|
|Wide range of (q)PCR products||Always a product to fit any thermocycler||Always best fitting plastics|
|Frosted plates and tubes||Higher signal/noise ratio||Lower copy detection|
|White plates and tubes||Highest signal/noise ratio||Low copy detection|
|Avoid signal contamination from block||Consistent reaction conditions|
|Breakable plates||Allow adjustment of required wells||Increased flexibility/lower cost|
|Low Profile tubes||Minimal evaporation||Consistent reaction conditions|
|Asymmetric holes in strips||Make your own plate||Easy transport and positioning|
|Optimal tube-block contact angle||Better heat transfer||Optimal reaction conditions|
|Alpha numeric markings||Easy sample location||Traceability samples|
|Auto-orientation holes||Easy orientation||Traceability samples|
|Frosted writing areas||Easy sample marking||Traceability samples|
|Laser embedded marking and coding||Easy sample identification||Traceability samples|
|Uniquely coded||Avoid mix-up samples & trays|
|Pipetting marker lines||Easy orientation|
|Extra robust strips||No twisting, wrapping or bending||Easy to handle, stable shape|
|In line and no breakage||Easy positioning and handling|
|Tear Off Format||Use of multiples of 8 wells||Economic usage|
|Pipette in 96 well format, use in 8 well or multiple of 8 well format|
|Removal of individual rows of a plate||Minimize cross contamination|
|Compatibility with Shell Frame Grid||Enabling to make specific sub skirted plates||Enables specific qPCR cycler fit|
|Easy use in robotics|
|Combined compatibility use (eg. ABI & Roche) thermocyclers|
|Closure with Tear Off cap strip mat||Simultaneous closure of complete tube strip mat||Easy and fast handling|
|Possible to separate closed 8-Tube strips||Set-up in 96 well format and use in 8 strip format|
|Filtertips and Tips||Special blend of polypropylene||Anti-static tips||Maximal sample recovery|
|Tips non sticking for biomolecules||Low copy detection|
|Flexible non cracking||Good fit & no loss of sample|
|Natural and extremely clear||Easy view of contents|
|Easy soft-fit and release from pipette||Reduce RSI|
|Beveled Orifice (45° )||Improve sample ejection accuracy||Consistent pipetting conditions|
|Graduated||Quick volume check||Minimize incorrect settings|
|Extended length||Avoid pipette to tube contact||Minimize cross contamination|
|18 micron filter||Superior protection||Minimize cross contamination|
|Gradient filter||Increases protection barrier||Minimize cross contamination|
|Extended filter length||Increases protection barrier||Minimize cross contamination|
|Modular composed tip racks||Multi application useable racks||Environment friendly|
|Micro Centrifuge tubes||Special blend of polypropylene||Anti-static tubes||Maximum sample recovery|
|Easy opening & closing||Reduce RSI|
|Flexible non cracking||No loss of sample|
|Clever design cap (inner round)||No corners in “inner part”||Maximal sample recovery|
|Graduated||Quick volume check||Helpful to avoid incorrect volumes|
|Screw Cap Tubes & Caps||Special blend of polypropylene||Anti-static tubes||Maximum sample recovery|
|Easy opening & closing||No loss of sample|
|Wide applications range & temperature||No need for cryo tubes|
|Clever design cap||Absence of rubber O-ring||No leakage or breakage|
|Suitable for organic solutions work range -200 °C to 100 °C|
|No corners in “inner part”||Maximum sample recovery|
|triangular shaped cap||No rolling of tubes|
|Colored tubes and caps||Color catch of tube and cap||Minimize cross contamination|
|Work and Cryo Racks||Special blend of polypropylene||One hand opening & closing||Increase flexibility|
|Flexible non cracking||No loss of samples|
|Wide applications range & temperature||No need for “special” cryo racks|
|Clever design||Modular composed racks||Multi application usage|
Controllability of (q)PCR
Accurate and reproducible results in (q)PCR are obtained when being in control of all variable parameters of this
process. So being in traceable control of these parameters such as reagents, oligonucleotides, template DNA,
disposables and thermocycler, next to other operational parameters, is crucial in achieving trustworthy
BIOplastics BV and its sister company CYCLERtest BV, both belonging to the Dutch BIOzym Holding BV group,
targeted these two, most often underestimated variables: The (q)PCR disposable and the (thermal) performance
of the (Real-Time) thermocycler.
CYCLERtest BV offers multi-channel thermal calibration equipment and ISO 17025 accredited calibration
services for any brand and model (q)PCR thermocycler. For more information about thermocyclers calibration
tools and calibration services: www.cyclertest.com, www.driftcon.com, www.celsiuslabs.com or call+31(0)45
BIOplastics BV manufactures Extreme Uniform plastic disposables, enabling control of the plastic consumable
aspect of the (q)PCR process. BIOplastics focuses on (q) PCR plates, strips and tubes. For customers who require
lowest variability, the number of the to be used products can be reduced to a minimum of two. Next to that
BIOplastics manufactures products used in the pre- and post-(q)PCR process; more specifically filtertips, pipette
tips, sampling tubes, racks, (cryo) storage tubes and racks, kit content reactions vessels and micro centrifuge
Extreme Uniform Plastics
To control your (q)PCR in the best possible way and to obtain the most reproducible and accurate results, BIOplastics developed a state-of-the-art range of (q)PCR disposables called: Extreme Uniform (q)PCR plastics. These plastics are characterized by Extreme Uniformity in:
- Wall thickness
- Low evaporation rates
- High thermal conductivity (if applicable)
- Low binding
- High liquid retention (if applicable)
- High signal to noise ratios
- Full interchangeability between used products (fit, binding and chemistry)
These characteristics lead to extreme uniform reaction conditions in (q)PCR, whereas the latest BIOplastics development enables to reduce the number of “different” products to a minimum of two, regardless the wide variety of brands and models in a laboratory.
BIOplastics manufactures not only Extreme Uniform (q)PCR disposables but has reduced, for those who require lowest variability, the number of to be used products to a minimum of two.
Interchangeability of products and processes
BIOplastics has assembled a range of products which are fully interchangeable with each other.
A (q)PCR process optimized in one of the BIOplastics strips, tubes or plates can simply be transferred to any other BIOplastics strip, tube or plate. The fact that BIOplastics offers products for ALL BRANDS and models of (q)PCR cyclers enables end-users to “one time only” optimize the (q)PCR process in its, at that moment in time, preferred BIOplastics model. Once optimized you can simply swap to a different BIOplastics format without re-optimizing or re-validating the process, since you are benefiting from our identical and interchangeable model and raw material properties plastics with the same properties. Using the same protocol on a different cycler is easily done by selecting the designated BIOplastics product for that model of cycler.
No need for re-optimizing the whole process since BIOplastics’ plastics characteristics are exactly the same for the whole range of the (q)PCR product line. Thermal performance difference between cyclers, cycler models, and brands can be accurately determined using CYCLERtest calibration service or CYCLERtest DRIFTCON® temperature calibration tools. See also “Normalizing Standard Operation Procedures (SOP) for (q)PCR applications” (page 22). Did you optimize your (q)PCR process in tube strips and need more or less capacity? Just start using BIOplastics plates, single tubes or even 384 well plates without the requirement of adjusting your protocol.
Shell Frame Grids
Shell Frame Grids are designed to combine designated BIOplastics (q)PCR vessel formats with an interchangeable and rigid skirt. This smart design enables to reduce the total amount of used plates or strips: to a minimum of 2, regardless the variety of your (q)PCR cycler range.
- Simply make your selection of low profile (0.1 ml) and/or regular profile 0.2 ml.
- Select your preferable format e.g. cut-able plate, tear-off strip mat, 8 tube-strips or 8 tube-strip with attached caps.
- Decide your preference for Ultra Clear, Frosted or White.
Your selected product(s) enable you to perform any (q)PCR on any (q)PCR cycler platform regardless cycler-range and assortment.
Outcome: superior accurate, uniform and interchangeability of results and limiting variables in MDX or experiments.
Saving costs on reagents
Low evaporation properties enables (q)PCR in low volumes which greatly reduces reagent costs (up to 60%).
BIOplastics does not recommend its products to be used with volumes below 5 µl.
White plates and strips do allow significant signal enhancement in qPCR. In some cases the reagent usage can be reduced due to the much higher signals achieved.
One (q)PCR disposable only to fit your cycler assortment
Why continue to use a number of different products to equip your (q)PCR cycler assortment if a “one product does it all” is at your fingertips. The use of BIOplastics’ Shell Frame Grid fit-able products as Tear Off Strip Mat
cut-able Plate are designed for “one product only” strategy which enables you to equip your whole 0.1 ml or 0.2 (q)PCR cycler assortment with one product only.
Reduce the number of products and vendors.
“One product does it all” BIOplastics (q)PCR products are designed to fit the majority of PCR and qPCR cyclers and our latest Shell Frame Grid fitting products even enables reduction of your usual (q)PCR disposables selection to one or two, regardless the cycler platform(s). Benefit buying from one vendor, using one product and one chemistry strategy, and grant your laboratory and its customers superior and consistent results. Related products as filter and pipette tips, micro centrifuge tubes etc. can be added to support your consistent results as well as to focus on a one vendor/superior quality laboratory strategy. We recommend using the dynamic search engine at our website to find the correct product for your application(s), opt for “Shell Frame Grid fitting” products or use our website “current competitor product” to BIOplastics superior products converter. If not sure or too much of a hassle, just contact BIOplastics for the best recommendation suiting your needs.
Eliminate ID# errors by irreversible coding
BIOplastics’ BPLPM technology results in a non removable, uniquely marked and coded product. No writing with markers, no mistakes, no removal of marks, no double identification numbers, just use the unique ID# at the beginning of your process. Link the unique ID to your Lab LIMS system. BPLPM technology is currently used in a selected range of products. BPLPM technology is particular useful for accredited labs and in Pre-diagnostics and Diagnostic settings allowing improvement of procedures and reducing risks of label
failures. Custom layouts and customized codes, whether in 1D or 2D can be added on demand. Several options (including your own coding) are available and particularly useful for kit manufacturers, enabling full traceability of products and applications.
Programmable, scannable and readable RFID chip
Chip incorporated (q)PCR strips and plates(RFID)
Molecular Diagnostics requires traceability and BIOplastics already has full trace ability for its products. Designated products are even unique and irreversible ID-ed and coded.
For those customers who would like to go beyond, BIOplastics enables the possibility for incorporating a programmable, scan able and readable chip (RFID) in its products. RFID or Radio Frequency Identification refers to a system for automatically identifying objects. The easiest way to understand RFID is to imagine a type of BARCODE which is able to exchange information by radio, and update itself over a period of time. In the coming years we expect lots of innovative applications with RFID. BIOplastics products with an RFID chip might be particular interesting for monitoring and streamlining workflows, diagnostic kits, diagnostic testing, combining instruments and applications or other parameters. Contact BIOplastics for information and options.
Avoid orientation mistakes
BIOplastics strips and plates are not symmetrical; many BIOplastics strips have off-center holes at top and centered holes at the bottom which provides easy orientation. Tubes strips and plates can be clicked in Shell Frame Grids permitting the assembly of your own plate or to perfectly fit your specific instrument. Frosted marking areas enable easy marking of strips. Extra robust strips will not break and enable straight forward positioning of the strips into the cycler. Strip caps are available in extra robust formats and allow easy positioning, opening and closing. Indented optical areas reduce “touching” the optical area while closing.
Reduce CO2 emission, environmentally friendly plastics
We at BIOplastics are, as many people, concerned about the environment in relation to the use of plastics. BIOplastics is often contacted in the assumption to have the solution for biodegradable-degradable plastics as these are also called “bioplastics”. At BIOplastics, we do monitor the development of these biodegradable plastics carefully and are open for incorporating these plastics into our product portfolio. The current technology and the type of applications, typically (q)PCR, does not (yet) allow us the use of biodegradable plastics. However to reduce the unnecessary pollution of our environment, BIOplastics has designed products that are interchangeable and can be use in a different lab application after serving the primary function. Some examples: filtertips and tip racks can be reused as (freezer) storage containers for tubes, plates and strips. You can use the rack to “pipette on ice” etc.. Furthermore the latest Tear Off plate mats and cap mats provide highest efficiency while very costs effective. Products fit able Shell Frame Grids offers superior reproducibility of results and allow using a minimize amount of product.
Standardize and improve reproducibility
BIOplastics manufactures the widest range (q)PCR disposables globally. We offer products for ALL BRANDS of PCR and qPCR cyclers and integrate full interchangeability between these products. (see also “Interchangeability of products and processes”). No need for shopping at different cycler manufacturers or vendors who are typically not the manufacturer. Enjoy the benefits and ease of use of superior quality (q)PCR vessels which all have the same raw material compositions. Combine this with the extreme uniform wall thickness of the products and you actually have standardized and improved the reproducibility of your results by excluding variables in uniformity, material composition and properties.
Reduce cross contamination, color coded screw cap tubes
Unlike other screw cap tube (SCT) manufacturers, BIOplastics manufactures not only natural screw cap tubes but also colored screw cap tubes. Reduce possible cross contamination in your lab by only using color matching tubes and caps. Ultimately one can opt using 1 or 2D irreversible bar-coded products, as e.g. screw cap tubes, our custom coding service or even laser code designated BIOplastics products by yourself.
Low copy detection
BIOplastics customized medical polypropylene blends are designed for low copy DNA, RNA and protein molecule detection through low binding characteristics. Polypropylenes are chemically inert, but still exhibit charged groups, static properties and hydrophobic areas. Different types or blends of polypropylene therefore do differ in binding characteristics and consequently charged groups like DNA, RNA, proteins and ions can bind in low amounts to polypropylene. BIOplastics (q)PCR products as well as pipette tips and micro centrifuge products are developed with a specific blend of PP. The blend is designed to maximize product functionality and application while providing extremely low binding characteristics to DNA, RNA and proteins. Due to these extremely low binding PP properties, products are superior for detecting low copy numbers of DNA, RNA and proteins. This blending enables reagent components such as ions and enzymes to be solely used for the reaction process and prevents sticking to the vessel surface. The binding (sticking) ratio of charged molecules like DNA, RNA and proteins between BIOplastics optimized PP blend and regular PP is typically 1:7 (1 to 7). (See also Anti Static tips PIPETTE TIPS, why, how and when they become favorable, page 17).
Extreme low binding characteristics of BIOplastics’ polypropylene blend
Flat stackable non-nesting plates
All BIOplastics plates are flat when purchased, which allows not only easy removal from a stack but also easy pipetting, positioning and removal from cyclers. Plates are stackable in a way that they do not nest or stick together. This is appreciated by those who do not want to waste time and effort into de-stacking plates as well as those who require liquid handling automation without de-stacking failures. Some plates are cut-able, some are breakable, some have “Tear Off” features, and some are additionally also assigned to fit Shell Frame Grids. Most plates have 12 centered and 12 non-centered holes which provide easy orientation for a plate or parts of a plate if it is cut or torn off. BPLPM technology provides “in product” permanent black markings (A-H, 1-12) as well as uniquely permanently coded plates (Number and/or 1D and/or 2D Laser-mark).
Ease of use
As a manufacturer, BIOplastics’ focus has been to design products with superior primary functionality, and
where possible, add ease of use and alternate use into the products. Below are several ease of use ideas.
Non nesting plates, ideal for pipetting and robotic applications
One hand closure of Single Tubes
Single tubes can be easily opened and closed by only using one hand. Single tubes can be placed in a BIOplastics Shell Frame Grid. The grid has functionality as a transport medium to and from cyclers and can be positioned into the cycler. The X - Y format remains the same avoiding requirements of individual tube labeling!
High molecule recovery, by product design
The design of the caps of BIOplastics tubes is different when compared to other supplier’s caps. To prevent any residue settling in the cap, regardless opened or closed, BIOplastics designed all its caps without any grooves, notches or cavity numbers. This design increases the recovery of liquid and its containing molecules. Furthermore, by nature of the design, liquid will automatically “drip down” to the bottom if the lids are in their closed position. Combining the above mentioned design features with BIOplastics polypropylene optimized blend properties, one ends up with maximum product functionality while providing extremely low binding characteristics to DNA, RNA and proteins and so highest molecule recovery.
Easy one hand opening and closing of single tube
Average wall thickness of 0.30 mm for tubes and strips and 0.35 mm for plates
Extreme uniform moulds
In the design and development stage BIOplastics pays considerable attention to a strategy that provides maximum mould and manufacturing accuracy. We outline precise product specifications, minimize mould cavities and define product thresholds, resulting in superior and reproducible products with, in the case of (q)PCR vessels, an average wall thickness of 0.30 mm for tubes and strips and 0.35 mm for plates both with a maximum tolerance of 0.05 mm.
Injection moulding mould
To manufacture qPCR products, tips and tubes with the highest uniformity possible, a low-cavity mould should be used. This means only a few products are moulded during one cycle, after which the next few are moulded. Manufacturing from low cavity moulds is more time consuming, and is therefore more costly than using high multi-cavity moulds from which 32, 64 or even 128 products are released in one cycle. Competitor’s products are often manufactured using high-multi-cavity moulds and therefore differ severely in tolerances and quality due to this production parameter. Manufacturing parameters of low cavity moulds are better controllable versus high cavity moulds. Injecting the polymer into a high multi-cavity mould (pressure/temperature) increases mould imbalance issues and consequently product inconsistencies. This can result in batches of
products with extreme differences in wall-thickness, closure, orifice and fit, which will influence the reproducibility of your experiment. BIOplastics products are manufactured using single, low- and semi-low cavity moulds.
injection moulding mould
Extreme uniform manufacturing conditions
During the injection moulding process all physical, chemical and mechanical parameters are fine-tuned to obtain an Extreme Uniform plastic. Our superior in-house expertise allows us to determine the optimum balance between injection temperature of the polypropylene, the temperature of the mould itself during the cooling process, the injection time and the shrinkage time. We benefit from the knowledge of our sister companies CYCLERtest and GENO-tronics both well-known as manufacturer of the MTAS® and Driftcon® systems as well as provider of ISO17025 accredited (q)PCR cycler calibration (On-Site) services. BIOplastics has incorporated modified temperature multi-sensor systems to monitor and control moulds and injections moulding machines. The manufacturing process takes place in a dedicated cleanroom under GMP and hands-free conditions to ensure the absence of detectable levels of DNA, RNA, DNase, RNase, proteins, pyrogens and ATP.
Granules of medical grade polypropylene
Extreme uniform raw material blends
The polypropylenes used are of the highest medical grade polypropylene, which ensures no release of metals or other contaminants into the reaction mix. The carefully selected types of medical grade polypropylenes are “in house blended” to well-defined proprietary ratios. This specific fine tuned blend supports achieving the highest product and user performance properties. BIOplastics avoids the use of softeners, coatings and mould releasing agents.
Non cracking (q)PCR tubes, strips and plates
The careful selection of different types of polypropylenes combined with extreme uniform wall thickness benefits BIOplastics products in a way that they do not crack easily. BIOplastics products can therefore withstand mechanical pressure and are impervious to possible small, microscopic, hairline cracks not visible with the naked eye.
So fact: EU products are strong, flexible and reliable and do not crack and are to easy handle!
Cross section of a (q)PCR tube
Extreme Uniform wall thickness leads to even heating of the sample and more homogenous reaction conditions, leading to more reproducible results. Extreme Uniform wall thickness also leads to lower evaporation rates and therefore more consistent (q)PCR results. In case of optical detection by optical reading through side of the tubes, an extreme wall uniformity increases signal consistency.
Regular Profile Tube
Low Profile Tube
Wall thickness and gas tightness
Polypropylene (PP) is not completely gas tight and evaporation through the walls is inevitable.
Sample loss through the wall is proportional to the wall thickness and is also related to the type of PP.
BIOplastics BV designs its (q)PCR vessels with thin walls at the bottom, to allow optimal maximum heat transfer, and thicker walls at the top to minimize evaporation through the PP which consequently leads to more reproducible results.
Thin Wall Thickness of BIOplastics products is defined as, and measured, 3 mm from the bottom, where as:
Single tubes and strips:
0.30 mm ± 0.05 mm
0.35 mm ± 0.05 mm
Tube angle, optimized for best fit
Different thermocycler brands and models have slightly different block well angles. BIOplastics uses its sister company’s (CYCLERtest) competence and knowledge of (q)PCR cyclers during the (q)PCR vessel design. BIOplastics (q)PCR vessels features a tube angle optimized to fit all main brands and models of (q)PCR thermocyclers, thus allowing maximum heat transfer for superior optimal and reproducible results.
Low, regular and high profile products
In low profile tubes there is a smaller “air” volume above the reaction mix than in regular and high profile tubes. This allows less reaction mix to go into gas phase, leading to more concentration of the samples, less change in reaction conditions and therefore more reproducible results. Fast (q)PCR cyclers accept low profile, also called 0.1 ml tubes, strips and plates. Regular (q)PCR cyclers accept regular profile products whereas (q)PCR cyclers with height adjustable lids accept low, regular and high profile products (tubes, strip-tubes and plates).
Easy opening and closure; cap design
Clever design and sealing bands around the tube closure points provide a leak-free seal, reducing evaporation and leading to more reproducible results. The superior sealing properties allows running (q)PCR reactions with volumes as low as 5 µl. The cap design allow minimum pressure for closure and opening which is highly appreciated by users and minimizes RSI risks.
Required strips can be easily torn off from the Tear Off Strip 8-Tube Strip Mat
Tear Off 8-Tube Strip Mat
The Tear Off 8-Tube Strip Mat can be considered as a flexible plate composed of 12 robust 8-tube strips which are connected to each other by thin bands. Part of the “plate” or one of multiple robust 8-tube strips can be easily and effortlessly torn off. Enjoy the benefit of full flexibility and use the format required for your application or cycler. Tear Off 8-Tube Strip Mats as a whole or in partitions can be “clicked in” Shell Frame Grids. Why not use one format only and enjoy the benefit of full flexibility.
Indented qPCR Cap, the optical area is recessed into the optical cap surface to prevent “touching” the optical area.
qPCR caps and indented optical areas
The caps of EU tubes and cap-strips are designed to enable fluorescent signals to pass through to the optical detection unit of a Real-Time thermal cycler. For the closure of EU strips and plates BIOplastics offers a range of EU wide optical area cap strips along with the “Opti-Seal” adhesive seals. The EU optical caps strips have
maximum optical areas (12.6 mm2
) and the thickness of the “optical lens” is reduced to 0.30 mm, which minimize light absorption by the plastics. BIOplastics’ designers have positioned the “optical area” indented in the cap surface which prevents “touching” the optical area during the whole (q)PCR process. EU wide area optical cap strips can be used on any of the (q)PCR tubes, strips and plates. The robust design prevents any deformation; applying and removing these cap-strips is easy.
Basic structure of polypropylene (PP)
Type and blend of polypropylene, low binding characteristics
Although polypropylenes are chemically inert, they still exhibit charged groups, static properties and hydrophobic areas. Different types and blends of polypropylene therefore show differences in binding of ions like magnesium, proteins, DNA and other charged groups which may influence your results. BIOplastics offers 3 different blends of polypropylene, these being O-type, A-type and M-type material. The O-type material is an optimal material used in (q)PCR grade reaction vessels. O-type material is composed by blending multiple polypropylenes specifically designed for (q)PCR applications. A-type material resembles the classic polypropylene mix, has better chemical resistance properties when compared to O-type and is available for some of the (q)PCR reaction vessels. M-type material is a more robust material which is mainly used for
0.5 ml, 1.5 ml and 2 ml tubes and pipette tips.
For (q)PCR applications we recommend O-type material for optimal results, as it is non-binding and shows the highest thermal conductivity. O-type and M-type material blends are specifically selected for non-binding characteristics to DNA, RNA and proteins.
Indelible in product labeling of a 384 well plate using BIOplastics’ BPLPM technology
In product coding and labeling with BIOplastics BPLPM technology
BIOplastics innovated the incorporation of micro particles in (q)PCR and related products.
These BPLPM technology (BIOplastics particle mix) products are offered in a majority of (q)PCR products. BPLPM enables indelible IN PRODUCT labeling and identification. While others use stickers, ink, stamps, or dyes containing organic solutions, BIOplastics’ BPLPM technique results in a irreversible, non-removable, uniquely marked and coded product. No writing with markers, no removal of marks, no double identification numbers; just use the unique ID#, barcode or 2D code at the beginning of your process. Link the unique ID#, barcode or 2D code to your Lab LIMS system and samples. BPLPM technology is particularly useful for accredited labs in MDX and reduce risks of label mistakes and failures. Custom layouts and customized codes can be requested and applied to the required products by BIOplastics or even applied by your own institute if correct laser mark units are available. These types of unique coding prove to be appealing for (MDX) kit manufacturers to effectively trace products and applications. See also Chip incorporation (RFID)
High Resolution Melting Curves (HRM) and regular melting curves
BIOplastics’ focus ensure maximum mould and manufacturing accuracy with precise product specifications, resulting in superior and reproducible products. BIOplastics’ (q)PCR vessels have a maximum tolerance of 0.05 mm. These extremely tight tolerances lead to extraordinary uniform and reproducible products.
Consequently, reproducible melting and high resolution melting curves, typically used in the post qPCR process, are generated using BIOplastics’ extremely uniform products. HRM curves can be normalized, i.e. “cycler fingerprint” adjusted, by using CYCLERtest® ISO17025 accredited cycler calibration services and calibration tools (www.cyclertest.com). See also page 151.
Shift of melting curves of one amplified fragment in qPCR. Shift caused by difference in qPCR plates as well as un-uniformity of the qPCR cycler
Gradient 18 micron filter with extended airflow and balanced optimal aerosol protection
Gradient filter in filtertips
The filter in a filtertip eliminates the formation of aerosols in the shaft of the pipette, since it blocks the air-to-liquid interface between the sample and the pipette shaft. This simple idea has evolved in numerous types and brands of filtertips and filter materials. Most filters are made of 3-dimensional cross-linked HMPE (Polyethylene), which is totally inert. The pore size should be smaller than 25 microns to generate a “reasonable protection” and not smaller than 14 microns to still allow an accurate airflow required when pipetting. The filtering capacity of a certain filter is defined as the ratio of filter length and pore size. The longer a filter is, the better it filters. This relation is almost linear. BIOplastics filters are the longest available in the market with a superior 18 micron pore size gradient whereas other suppliers use shorter length and 25 to 30 micron filters! By applying a gradient into the filter, BIOplastics’ filtertips have an extended airflow and a balanced optimal aerosol protection due to its pore size and filter length, with a minimum compromise of pipetting accuracy.
BIOplastics tips have a very fine orifice for complete, reproducible pipetting. The pipette tips are extremely clear to allow content examination if required. Tips are extremely uniform, flexible and soft, to secure a good leak-free fit around with the shaft of the pipette. The soft, ultra-clear medical grade material also reduces RSI
since the force to attach and release the tip from the pipette is significantly reduced. The fine orifice assures reproducible pipetting. Tips marked with the beveled orifice icon have a special 45° beveled orifice to guide the ejection of the fluids even better. Furthermore the raw material used in pipette tips has very low binding
properties to biomolecules and has superior retention/recovery properties when pipetting ion-containing solutions.
Screw Cap Tubes (SCT)
BIOplastics’ smart secure closure technology results in superior screw cap tubes. The screw caps are designed in a way that the use of “old fashioned” rubber rings has become obsolete. The absence of a rubber ring assures that the closure is not affected when in contact with organic solvents nor that leakage occurs due to hardening and unbalanced shrinkage caused by low and high temperatures and pressure. The smart secure closure design allows frequent opening and closing, even in extreme conditions, without compromising the closure and also avoiding leakage (working range -200 °C -+100 °C). Screw Cap Tubes are available in low binding and extreme robust formats which even allow severe bead beating procedures.
Pipette tip with fine orifice for complete, reproducible pipeting
Unique coded tubes, strips and plates
Due to BIOplastics’ BPLPM technology, products can be individually and uniquely coded. These products are available for the qPCR product range as well as for micro centrifuge tubes and screw cap tubes. Each tube can have a unique ID#, 1D or 2D code. Specific codes or customized marking of any BIOplastics product are available on demand.
qPCR signal enhancement White, frosted products and ultra clear products
A number of qPCR cycler brands and models are available in the marketplace. Although they have a lot in common they also differ from each other. There are two major differences a userhas to understand in making the best choice in the disposables as well for generating superior results.
The two major differences are:
- Some brands are using low profile (LP) also called 0.1 ml plates/tubes instead of regular profile (RP) 0.2 ml plates/tubes
- Most cyclers do their optical reading from the top of the plates/tubes, while some do the optical reading through the side of the plate/tubes
For differences in low profile (LP) 0.1 ml plates/tubes, regular profile (RP) 0.2 ml plates/tube see section “Differences in Low Profile, Regular Profile and High Profile products” at page 16.
Optical reading through the caps
Optical reading through the caps
qPCR signal to noise ratios can be significantly increased by using frosted qPCR tubes, strips and plates.
Frosted products will generate higher signal to noise ratios (S/N ratios) up to 40% compared to transparent ones. By using frosted products both content visibility and high signal/noise ratio are achieved. White products are superior for enhancing signal to noise ratios. White products greatly enhance signal/noise ratios up to 90% compared to transparent ones and up to 50% compared to frosted products. Possible block pollution of dyes, differences in coatings, and inconsistent coatings can influence qPCR signals. White products will eliminate these interferences while frosted products will reduce the inconsistencies when compared to natural transparent products. Micro-particles, by nature, increase signal to noise ratios in Real-Time PCR applications. BIOplastics BPLPM technology incorporates micro-particles that increase signal to noise ratios in Real-Time PCR applications.
So optical reading through the cap:
Ultra Clear products
High S/N ratios
Moderate S/N ratios
Poor S/N Ratios
White tube with transparent optical cap High S/N Ratios
Frosted tube with transparent optical cap Moderate S/N Ratios
Transparent with transparent optical cap Poor S/N Ratios
Comparison of Real-Time signals when using Ultra Clear, Frosted and white BIOplastics products.
Based on (q)PCR optical measurements through the cap.
Optical reading through side of the Tubes
Optical reading through side of the Tube
While many cyclers use the principle of optical reading through the cap, some cyclers generate an optical detection by optical reading through the sides of the tubes. In this case the highest signals are achieved using Ultra Clear (UC) transparent qPCR tubes, strips and plates. Preferably one needs the ultra clear feature at the conical part of the tubes. Beneficially the straight upper part of the tube could be frosted, where as the use of a white cap provides an additional increase of signal to noise ratios (S/N).
Frosted products will generate lower signal/noise ratios compared to Ultra Clear transparent ones.
White caps are superior for enhancing signal/noise ratios compared to transparent ones. White caps enable reflection of “lost fluorescence” resulting in increased signals.
So Optical reading through side of the tube:
Ultra Clear Conical part of tube and white caps
Ultra Clear Conical part of tube and caps
Frosted tube products
Highest S/N ratios
High S/N ratios
Moderate/poor S/N ratios
Ultra Clear tube with white cap
Ultra Clear tube with Ultra Clear optical cap
Frosted with frosted cap
The difference between low, regular and high profile strips
Differences in Low Profile, Regular Profile and High Profile products
Although low profile tubes were introduced in the marketplace back in 2000, some companies have renamed the original low profile 0.2 ml vessels description to 0.1 ml “vessel”. Low profile (0.1 ml) products are used in “fast cyclers” from ABI, Roche 480 cyclers as well in (q)PCR cyclers with height adjustable lids. The difference
between low profile 0.1 ml vessels and regular profile products is the height of the product and consequently the volume it can hold. More than half of a regular-profile strip remains above the thermal block level. In low profile tubes there is a smaller “air” volume above the reaction mix than in regular and high profile tubes. This allows less reaction mix to go into gas phase, leading to less concentration of the samples, less change in reaction conditions and therefore to more reproducible results. Most fast cyclers accept low profile tubes, strips and plates. Most regular PCR cyclers accept regular profile and high profile file products. (tubes, tube-strips and plates). Thermal cyclers without height-adjustable heated lids accept only one type, mainly regular profile products. BIOplastics products are available in all profile versions. The differences between low profile (0.1ml), regular profile and high profile products are shown in the illustrations and photographs below.
Low Profile (LP), Regular Profile (RP), High Profile (HP), Fast Cycler, 0.1 ml, 0.2 ml tubes?
The industry did muddle the issue and customers are confused about "what is what".
Find the similarities and differences below:
Low Profile (LP)
Regular Profile (RP)
High Profile (HP)
= 0.1 ml tube/plate
= 0.2 ml tube/plate
= 0.2 ml tube/plate
height without closure: 15.6-16.3 mm
height without closure: 20.9-21.5 mm
height without closure: 22.5-24.0 mm
Raw material and product properties
The polypropylenes used are of the highest medical grade polypropylene, which ensures no release of metals or other contaminants into the reaction mix. The carefully selected types of medical grade polypropylenes are “in house blended” to well-defined proprietary ratios. This specific fine tuned blend supports achieving the highest product and user performance properties.
Table BIOplastics Raw material composition properties
|Type of vessel||Application||Competitor vessel||BIOplastics tubes (M type)||BIOplastics (O type)|
|(q)PCR tubes, strips, plates ||Binding to DNA % ||Up to 2% ||NA ||< 0.3% |
| ||Binding to proteins % ||Up to 4% ||NA ||< 0.8% |
| ||Temperature work range °C||mainly -20 to 100 °C||NA ||-30 to 100 °C|
|Microcentrifuge tubes 0.5, 1.5 & 2 ml||Binding to DNA % ||Up to 5% ||< 0.6% ||< 0.3% |
| ||Binding to proteins % ||Up to 6% ||< 0.6% ||< 0.6% |
| ||Pop-Open at 99 °C ||yes > 80% ||No ||No |
| ||Temperature work range °C||mainly -20 to 99 °C ||-80 to 100 °C ||-80 to 100 °C|
|Screw cap tubes 0.5, 1.5 & 2 ml ||Binding to DNA % ||Up to 5% ||< 0.6% ||< 0.3% |
| ||Binding to proteins % ||Up to 6% ||< 0.6% ||< 0.6% |
| ||Accept organic solutions ||No > 95% ||Yes ||Yes |
| ||Temperature work range °C||mainly -25 to 100 °C||-200 to 100 °C||-80 to 100 °C|
|Titer dilution and storage tubes ||Binding to DNA % ||Up to 4% ||< 0.6% ||NA |
| ||Binding to proteins % ||Up to 4% ||< 0.6% ||NA |
| ||Temperature work range °C||mainly -25 to 100 °C||-180 to 100 °C||NA |
The EU Gold Standard for (q)PCR
BIOplastics BV has set the EU Gold Standard for (q)PCR by being in control of all variables in the process starting from the principle design up to the final packaging, with products of superior performance relative to signal, closure, evaporation, and reproducibility.
Anti-static pipette tips. Why, how, and when they become favorable.
BIOplastics pipette tips are designed for use in molecular biological applications and more specifically for pipetting DNA, RNA, proteins and solutions commonly used in and around the (q)PCR process. BIOplastics has optimized its pipette and filtertips by means of design and raw material selection to meet the highest requirements. By selecting medical grade materials with anti-static properties BIOplastics has reduced the biological molecule binding to the lowest possible. BIOplastics pipette tips become favorable when pipetting slightly basic buffers, salt solutions and biological molecules (Proteins, DNA, RNA). Differences in hydrophobic and hydrophilic properties of solutions, raw material surface and biomolecules cause a pipetting difference “phenomenon” as shown below. If pipetting water contact us for regular “old type” tips.
Purity and certifications
Product purity is, along with excellent design and functionality of products, an important and solid foundation that enables customers to generate reliable, reproducible and trustworthy results. BIOplastics products are fully traceable and frequently tested during production. BIOplastics products comply with the highest standards and requirements. Polypropylenes are medical grade and fully incoming quality controlled, prior to use in our proprietary blending methodology. All injection molding process parameters, QC tests and packaging are monitored, logged and fully traceable. If required, BIOplastics will provide batch certificates as well as individual certificates.
Certificate of Analysis (COA)
All BIOplastics products are extensively process qualitycontrolled and tested. Our QC-laboratory does the final testing prior release to the warehouse. A Certificate of Analysis can be requested at the BIOplastics website. Each request for COA will be re-traced in our fully traceable system prior to COA release.
Traceability of products
BIOplastics has a fully compatible traceable system in place. This means that each bag can be traced from the incoming raw material through production to whom the product was sold. Traceability is 100% and based on more than 80 parameters.
Cleanroom, “no hands on” manufacturing
BIOplastics products are molded and automatically collected, inspected, quality controlledand packaged in a Class 100,000 cleanroom environment. Manufacturing, packaging, in process and final release QC, operate as independent entities, each with independent responsibilities. Appropriate body protection, including masks and gloves are mandatory for all departments.
BIOplastics’ company procedures are similar to ISO9001 and GMP procedures. Although we formally do not
have to carry an ISO9001 stamp we are scheduled to have an ISO9001 accreditation shortly.
Yellow bags, why?
Although BIOplastics manufactures all its injection molding products, we do not manufacture the bags where the products are packed into. They are manufactured by an external blow molding company. Although the bags are guaranteed clean we still take extra precautions. We therefore sterilize all packaging material, in particular “zip lock” bags by means of cobalt60
irradiated plastics may lead to a slightly more brittle bag as well as slightly yellow discoloration. (may vary based on amount of radiation and batch of bags)
Product is autoclavable when you see this icon
Autoclave when, how and why?
All BIOplastics products are autoclavable except the easy closure screw caps. Autoclaving instructions:
15 minutes at 121°C and 2 bar pressure. A lot people confuse sterile products with DNa(se), RNa(se), Protein and DNA free products. BIOplastics (q)PCR products are manufactured free of any detectable levels of DNa(se), RNa(se), proteins and DNA. So the products do NOT REQUIRE any additional treatment.
The AUTOCLAVING process DOES NOT REMOVE any DNA, RNA or proteins. It does however breakdown larger molecules and “kill” living organisms. So a sterile product is not necessarily a DNA, RNA free product whereas a DNa(se), RNa(se), Protein and DNA free product can by definition not be categorized as sterile.
Bottom line for all BIOplastics products in general: Use them as they come!
Co irradiation exposed sticker as sterility indicator
What is sterility?
A sterile product is a product which is free of any living micro-organisms. Sterilization of BIOplastics products is accomplished by means of cobalt60
irradiation. Irradiated products age faster than non-irradiated products; they are less flexible and break more easily, because cobalt60
irradiation slightly changes the characteristics of the polymer material. Autoclaving the product yourself is a more gentle process, but when done several times it will also harden the polymer. One could argue whether it is necessary to autoclave or irradiate BIOplastics (q)PCR disposables at all, since our products are produced under cleanroom and “no-hands-on” conditions.
See also autoclave when, how and why? (Page 18).
What is DNa(se), RNa(se) and pyrogen-free?
Producing products under strict cleanroom and “no-hands-on” conditions ensures the absence of DNA, RNA, and their breakdown enzymes. A sterile product is not necessarily DNA(se), RNA(se) and pyrogen-free.
Only regular testing during and after the production process guarantees a DNA(se), RNA(se) and pyrogen-free product. You can recognize the DNA(se), RNA(se) and pyrogen-free plastics in this catalog when you see the “Thumbs-Up” icon.
Human DNA and ATP absence?
Manufacturing products under strict clean and “no hands-on” conditions, as well as regular testing during and after the production process, ensures the absence of human DNA and ATP.
What is metal-free?
The absence of metals in polymer products like tips and tubes is essential for laboratory use, as metals can interfere in (q)PCR and other reactions with ions as Mg2+. BIOplastics tips and tubes are made of PP (Polypropylene) of the highest medical grade quality available and guaranteed metal free.
Example of product package label with individual bag ID
Why we do not work with lot#, but with individual trace ID# instead
Most companies work with so called Lot ID#(lot number). We at BIOplastics do not use the “lot ID” categorizations since raw material and time is involved in its definitions. Since we prefer to have more exact and precise data, we have narrowed the time factor down to a maximum range of 4 hours. It is for that reason that we use individual bag IDs which enable us to trace and isolate products to a manufacturing and packaging time frame of 4 hours maximum. Ultimately this is beneficial for you as a customer as it is for us.
While this has not yet occurred, in any particular case it would enable us to recall products to a production time of 4 hours. Since we can trace to whom we sold individual bags, we would be able to precisely isolate products without interfering or disturbing non involved customers.
BIOplastics is proud that the number of product complaints is very low. Furthermore 97% of claims ever received could be traced back to a user related issue. Let’s be clear: We at BIOplastics aim for 100% product satisfaction. We feel obligated to achieve this goal and have a team of application specialists available to help and investigate. Should you have a complaint, then we will ask you to fill out a complaint form including details of your experiments, as well as returning the products in question if possible. In all cases we will counter investigate and report our findings and recommendations back to you. If a claim is granted by our specialist teams we will replace the product free of charge. If a claim is not granted we will contact you and provide you with details on how to solve your problem. We believe this to be a fair, functional, and "no nonsense" policy.
Customized products, MDX kit manufacturers OEM requirements
Due to BIOplastics BV experience in developing and manufacturing of superior products and the recognition of an excellent quality to price ratio, tailor-made products have become an additional aspect of BIOplastics BV.
BIOplastics BV can be a partner in the field of designing and manufacturing of:
- Custom Laser labeling, barcoding, 2D coding
- Custom product Chip incorporation (RFID)
- Custom raw material adjustment
- Large scale OEM (Original Equipment Manufactured) products
- New products
- Customized design
Other customized solutions are possible:
- Different material characteristics
- Different product properties
- Different colors
- Kit content solutions
Customized solutions and products are based on BIOplastics BV core competencies and innovative technologies. Products can be designed according to your specific requirements and manufactured based on either exclusivity and/or intended to sell under client’s brand name.
Customized Coded products using BPLPM Technology:
- Any type of product related to BIOplastics’ current portfolio
Customized products and OEM requirements consultation:
Contact BIOplastics BV headquarters in The Netherlands or send an e-mail to OEM@bioplastics.com