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The difference between low, regular and high profile strips

Differences in Low Profile, Regular Profile and High Profile products

Although low profile tubes were introduced in the marketplace back in 2000, some companies have renamed the original low profile 0.2 ml vessels description to 0.1 ml “vessel”. Low profile (0.1 ml) products are used in “fast cyclers” from ABI, Roche 480 cyclers as well in (q)PCR cyclers with height adjustable lids. The difference between low profile 0.1 ml vessels and regular profile products is the height of the product and consequently the volume it can hold. More than half of a regular-profile strip remains above the thermal block level. In low profile tubes there is a smaller “air” volume above the reaction mix than in regular and high profile tubes. This allows less reaction mix to go into gas phase, leading to less concentration of the samples, less change in reaction conditions and therefore to more reproducible results. Most fast cyclers accept low profile tubes, strips and plates. Most regular PCR cyclers accept regular profile and high profile file products. (tubes, tube-strips and plates). Thermal cyclers without height-adjustable heated lids accept only one type, mainly regular profile products. BIOplastics products are available in all profile versions. The differences between low profile (0.1ml), regular profile and high profile products are shown in the illustrations and photographs below.

Low Profile (LP), Regular Profile (RP), High Profile (HP), Fast Cycler, 0.1 ml, 0.2 ml tubes?

The industry did muddle the issue and customers are confused about "what is what".
Find the similarities and differences below:
Low Profile (LP)
Regular Profile (RP)
High Profile (HP)
= 0.1 ml tube/plate
= 0.2 ml tube/plate
= 0.2 ml tube/plate
height without closure: 15.6-16.3 mm
height without closure: 20.9-21.5 mm
height without closure: 22.5-24.0 mm

Differences in Low Profile, Regular Profile and High Profile products Image2